DNA replicates by separating into two one strands, Just about every of which serves being a template for any new strand. The new strands are copied by the same basic principle of hydrogen-bond pairing amongst bases that exists inside the double helix. When DNA separates to replicate, DNA polymerase (and another enzymes) attach new bases to every

Strands are subject matter to hybridisation selection - considering that all oligos overlap totally, errors within their syntheses will create mismatches which imply They're less likely to anneal and type a ligated product or service. Due to the fact a small degree of incorrect bases at any place can not be observed through sequencing (it can be